THE COMPOSITION OF GOLD NANOPARTICLE CONJUGATES WITH IMMUNOGLOBULINS AT PHYSICAL ADSORPTION OR COVALENT BINDING

1 SOTNIKOV Dmitriy V.
Co-authors:
1 BYZOVA Nadezhda A. 1 ZHERDEV Anatoly V. 1 DZANTIEV Boris B.
Institution:
1 A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia, sotnikov-d-i@mail.ru, nbyzova@inbi.ras.ru, zherdev@inbi.ras.ru, dzantiev@inbi.ras.ru
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
446-450
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
53 views / 18 downloads
Abstract

A quantitative comparison was made of the efficiency of conjugation of immunoglobulins G and gold nanoparticles obtained by the Turkevich-Frens method. The conjugates were synthesized by physical adsorption and covalent immobilization using orthopyridyl disulfide polyethylene glycol succinimidyl ester (OPSS-PEG-NHS). The study is conducted using an original method of determining the composition of protein nanoconjugates based on measuring the intrinsic fluorescence of tryptophan in the protein. The percentage of bound immunoglobulins was higher when using OPSS-PEG-NHS: 43% versus 22% for adsorption conjugation at an added IgG concentration of 2.3 μg/ml. After increasing the IgG concentration to 4.6 μg/ml, the percentage of binding immunoglobulins was close for the two methods of the conjugation: 26% and 22%, respectively, which may indicate the filling of the surface of the particles.

Keywords: Gold nanoparticles, conjugates, tryptophan fluorescence, immunoglobulin
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