PREPARATION OF MAGNETIC POLYHYDROXYBUTYRATE MICROPARTICLES AND APPLICATION FOR INVERTASE IMMOBILISATION

1 BERAN Milos
Co-authors:
1 PINKROVA Jitka 1 DRAHORAD Josef 1 MOLIK Petr 1 VLTAVSKY Ondrej 2 SOVA Jiri
Institutions:
1 Food Research Institute Prague, Prague, Czech Republic, EU
2 RCMT, Czech Technical University, Prague, Czech Republic, EU e-mail: m.beran@vupp.cz
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
499-504
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
18 views / 4 downloads
Abstract

A new original technology of production of PHB hollow microspheres with addition of nanoparticular magnetite by carbon dioxide assisted nebulisation drying has been developed. Invertase enzyme (EC 3.2.1.26) was immobilised on the microparticles surface by a simple process exploiting hydrophobic interactions. The biocatalyst had very good activity and stability after repeated applications for sucrose hydrolysis to glucose and fructose. It can be used in effective biocatalytic production of inverted sugar syrup.

Keywords: Invertase; immobilisation; magnetic microparticles; polyhydroxybutyrate; spray nebulisation
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