A new original technology of production of PHB hollow microspheres with addition of nanoparticular magnetite by carbon dioxide assisted nebulisation drying has been developed. Invertase enzyme (EC 18.104.22.168) was immobilised on the microparticles surface by a simple process exploiting hydrophobic interactions. The biocatalyst had very good activity and stability after repeated applications for sucrose hydrolysis to glucose and fructose. It can be used in effective biocatalytic production of inverted sugar syrup.Keywords: Invertase; immobilisation; magnetic microparticles; polyhydroxybutyrate; spray nebulisation
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