Here we report the optimal conditions for the isolation of reduced glutathione (GSH) and oxidized glutathione (GSSG) from various types of matrix, such as blood, plasma or cells and their characterization using ion-exchange liquid chromatography with VIS detector. In our study, we aimed to synthesize paramagnetic microparticles able to isolate and immobilize GSH, GSSG from blood or plasma and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified amberlite and Dowex by nanomaghemite (γ-Fe2O3), as the functional carriers providing perfect paramagnetic properties. Both the paramagnetic microparticles were able to bind GSH and GSSG. Our paramagnetic microparticles have potential to improve GSH and GSSG isolation and in future can serve for application as a platform of biosensors or delivery system.Keywords: Ion Exchange Chromatography; Maghemite Microparticles; glutathione; reactive oxygen species
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