Nowadays, nanoparticles (NPs) are enjoying unprecedented popularity due to their use in various applications. Even despite the enormous benefits of those man-made entities in numerous applications, many serious impacts on human health and the environment have been reported. To study NPs intrinsic biological properties, we chose a cathode sputtering for their preparation eliminating the use of toxic solvents and reducing agents. According to the transmission electron microscopy and dynamic light scattering measurements, we prepared non-agglomerated faceted NPs with diameter of 1.8 0.4 nm and 2.3 0.4 nm for platinum and palladium, respectively. We evaluated their cytotoxic activity by WST-1 assay using human embryonic kidney (HEK 293T) cell line. We also determined the antibacterial properties of these NPs against two common pollutants (E. Coli, a Gram-negative bacteria and S. epidermidis, a Gram-positive bacteria).We observed appreciable toxicity of metal NPs in all studied samples.Keywords: Sputtering, metal nanoparticles, glycerol
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