ELEGANT, RAPID AND SIMPLE PROCEDURE FOR LABELING OF ANTIBODIES WITH FLUORESCENT ZNCD/S QUANTUM DOTS FOR LASER ABLATION ICP-MS DETECTION

1,2 ASSI Navid
Co-authors:
1,2 PAVELICOVA Kristyna 1,2 VACULOVICOVA Marketa 3 VACULOVIC Tomas
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic, EU, navid.assi@vutbr.cz, marketa.ryvolova@seznam.cz, k.pavelicova@seznam.cz
2 Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic, EU, navid.assi@vutbr.cz, marketa.ryvolova@seznam.cz
3 Department of Chemistry, Masaryk University in Brno, Brno, Czech Republic, EU, tomas.vaculovic@ceitec.muni.cz
Conference:
12th International Conference on Nanomaterials - Research & Application, Brno, Czech Republic, EU, October 21 - 23, 2020
Proceedings:
Proceedings 12th International Conference on Nanomaterials - Research & Application
Pages:
395-398
ISBN:
978-80-87294-98-7
ISSN:
2694-930X
Published:
28th December 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
125 views / 68 downloads
Abstract

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Keywords: Antibody, antigen, quantum dots, cleavage disulfide bonds, fluorescent

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Scroll to Top