THE STUDY OF ANTIMICROBIAL ACTIVITY OF AGNPS MADE BY USING GREEN SYNTHESIS AGAINST SPECIFIC MICROORGANISMS

1 Sochor Jiri
Co-authors:
1,2,3 SEHNAL Karel 4 Ruiz-Rico Maria 4 Perez-Esteve Edgar 1 Sochorova Lenka 1 Baron Mojmir 4 Barat Jose M. 1,2,3 KIZEK Rene
Institutions:
1 Mendel University in Brno, Faculty of Horticulture, Department of Viticulture and Enology, Lednice, Czech Republic, EU, tomaskova.l.9@gmail.com
2 Department of Research and Development, Prevention Medicals s.r.o., Tovární 342, 742 13; Studénka-Butovice, Czech Republic, karelsehnal15@gmail.com
3 Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, 612 42 Brno, Czech Republic, kizek@sci.muni.cz
4 Grupo de Investigación e Innovación Alimentaria, Departamento de Tecnología de Alimentos, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain, maruiri@etsia.upv.es
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
536-541
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
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Abstract

This work is focused on studying the antimicrobial effect of silver nanoparticles made by green synthesis. For the green synthesis, extracts from Salvia officinalis were used. Four different temperatures (20, 40, 60, 80 °C) were used to prepare the extracts from the plant material. The antimicrobial capability of the AgNPs was assessed using different yeasts (Saccharomyces cerevisiae, Zygosaccharomyces bailii), gram-positive (Lactobacillus plantarum, Listeria innocua, Staphylococcus Aureus) and gram-negative (Escherichia coli, Acetobacter aceti) bacterial strains. The range of AgNPs concentrations 0–2,500 µg/mL were tested. The antimicrobial effect was studied using two methods: 1) the viable cell count method and 2) the inhibition zone method. The method of direct counting showed a small inhibitory effect (inhibition at concentrations 1,250 and 2,500 µg/ml) only for E. coli, Z. bailii and S. cerevisiae. The inhibition zone results displayed better antibacterial activity of the silver nanoparticles (inhibition at all concentrations - 50, 100, 150 and 200 µg/mL) prepared by using the extract obtained at 20 ºC against E. coli and S. aureus.

Keywords: green synthesis, silver nanoparticles, antimicrobial activity
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