This pilot study deals with the detection of metal-based solid particles in the human ejaculate and their possible connection with infertility. Ten samples provided by cytogenetics department, CGB Laboratory, a.s. in Ostrava – Vítkovice, were analysed by scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDS) and Raman microspectroscopy. Metal-based particles, particularly those containing iron, aluminium, copper, zinc, chromium, titanium and nickel, were detected in the samples. At the same time, titanium compounds and calcium carbonate were found. Many of these substances have already been shown to have a negative effect on the living organism. Based on the information found, there might be some dependence between the presence of solid metal particles in the ejaculate and male fertility impairment.Keywords: Male infertility, metal based solid particles, scanning electron microscopy, Raman microspectroscopy
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