GREEN SYNTHESIZED QUANTUM DOTS AS ELECTROCHEMICAL LABELS FOR SENSITIVE DETECTION OF HEMORRHAGICHAEMORRHAGIC FEVER VIRUS

1,2 HOSNEDLOVA Božena
Co-authors:
2,3 SEHNAL Karel 2 TOTHOVA Zuzana 4 KEPINSKA Marta 3 RUTTKAY-NEDECKY Branislav 5 FERNANDEZ Carlos 6 OFOMAJA Augustine Enakpodia 4 MILNEROWICZ Halina 1,2,3,4 KIZEK René
Institutions:
1 Department of Food and Feed Safety, Veterinary and Research Institute, Hudcova 70, 621 00 Brno, Czech Republic, bozena.hosnedlova@post.cz
2 Department of Research and Development, Prevention Medicals s.r.o., Tovární 342, 742 13; Studénka-Butovice, Czech Republic, uhlirova@preventionmedicals.cz
3 Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, 612 42 Brno, Czech Republic, kizek@sci.muni.cz
4 Department of Biomedical and Environmental Analyses, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland, zalewska.m@gmail.com
5 School of Pharmacy and Life Sciences, Robert Gordon University, Garthdee Road, Aberdeen, AB10 7QB, Scotland, United Kingdom, c.fernandez@rgu.ac.uk
6 Biosorption and Wastewater Treatment Research Laboratory, Department of Chemistry, Faculty of Applied and Computer Sciences, Vaal University of Technology, P. Bag X021, Vanderbijlpark, 1900, South Africa, augustineo@vut.ac.za
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
416-422
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
59 views / 15 downloads
Abstract

Virus-induced haemorrhagic fevers often show a rapid, dramatic course. Infectivity and mortality are very high. Such viruses are known to attack primates as well as other animal species. Fast, sensitive, selective diagnostics can be very beneficial for early anti-epidemic measures right in the outbreak. Quantum dots (QDs) have excellent features that make them useful as detection labels. Their use increases the sensitivity of the analytical assay. The use of biologically active components in QDs synthesis brings new properties. Quantum dots are nanocrystals that due to their high stability, ease of preparation and biocompatibility are suitable for labelinglabelling biomolecules. CdTe QDs modified with mercaptosuccinic acid (MSA), reduced glutathione (GSH), and plant extracts were prepared. Green synthesized CdTe QDs showed emission depending on the amount of extract applied. Thus, QDs were prepared in full-color scale from blue to red. The modified biomolecules of QDs are applicable for the labelinglabelling of nucleic acids. Their long-term stability, including their application onto paper, was tested. CdTe QDs exhibit very good electrochemical detection with the limit of detection (LOD) in nanomolar concentration.

Keywords: Dangerous viral diseases, QDs, green synthesis, viral infection, CdTe, sensors, electrochemistry, electrochemical biosensor
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