HIGHLY SELECTIVE AND SENSITIVE ELECTROCHEMICAL BIOSENSOR BASED ON ELECTROCHEMICALLY REDUCED GRAPHENE OXIDE FOR DETECTION OF MIRNA AS A CANCER BIOMARKER

1 VANOVA Veronika
Co-authors:
1,2 SEDLACKOVA Eliska 1,2 HYNEK David 1,2 RICHTERA Lukas 1,2 BYTESNIKOVA Zuzana 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno, CZ-613 00, Czech Republic, EU, veronikavanova15@gmail.com 
2 Central European Institute of Technology, Brno, University of Technology, Purkynova 123, Brno, CZ-612 00, Czech Republic, EU
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
385-390
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
69 views / 15 downloads
Abstract

Nowadays, cancer is one of the most outrageous diseases affecting millions of people throughout the world. In this study, the sensitive biosensor for miRNA-21 detection was developed. Through a facile sulfonamide coupling reaction a miRNA-21 specific DNA hybridization probe was immobilized onto a glassy carbon electrode (GCE) modified with electrochemical reduced graphene oxide (erGO). miRNA is a small non-coding RNA molecule which plays an important role in gene expression. The level of miRNA in extracellular fluids varying in the presence of cancer and it can serve as creates a potential biomarker for early detection of cancer. A higher levelcancer. An elevated level of miRNA-21 represents a common feature of pathological cell growth or cell stress and is connected with almost all types of cancer and with cardiovascular diseases, as well.The aim of this study was improve the electrochemical properties by using erGO as a modification for better sensitivity of the developed biosensor. After the modification with erGO, 1-amino-2-naphthol-4-sulfonic acid (AN–SO3–) was electrodeposited on the GCE surface.Then the GCE was subsequently modified by electrochemical deposition 1-amino-2-naphthol-4-sulfonic acid (AN–SO3–) on the GCE surface. Finally, the amino terminated capture probe was immobilized on the GCE via the sulfonamide coupling reaction. The developed biosensor for the detection of specific miRNA sequence was optimized and modified for using in further experiments.

Keywords: Reduced graphene oxide, miRNA, biosensor, electrochemical detection
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