AN ELECTROCHEMICAL BIOSENSOR DEVELOPED FOR THE ONLINE MONITORING OF H2O2 BASED ON THE REDUCED GRAPHENE OXIDE-CERIUM DIOXIDE NANOCOMPOSITE

1,2 MUKHERJEE Atripan
Co-authors:
1,2,3 ASHRAFI Amirmansoor 1,2 BYTESNIKOVA Zuzana 1,2 RICHTERA Lukas 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno, CZ-613 00, Czech Republic, EU, atripan@gmail.com
2 Central European Institute of Technology, Brno, University of Technology, Purkynova 123, Brno, CZ-612 00, Czech Republic, EU
3 CEITEC-Central European Institute of Technology, Mendel University in Brno, Zemedelska 1, Brno, CZ-613 00, Czech Republic, EU
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
396-401
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
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Abstract

A biosensor was developed for monitoring H2O2 based on reduced Graphene Oxide-Cerium Oxide (rGO-CeO2) nanocomposite (NC). The NC shows catalytic effect on the electrochemical reduction of H2O2 at −0.3 V. The effect of the NC on the electrochemical reduction of H2O2 was studied by cyclic voltammetry and compared with bare glassy carbon (GC) and GC/r-GO. Moreover, to improve the signal a complex containing copper was incorporated in the dispersion of the nanocomposite which acts as a mediator. It was found that the electrochemical reduction of H2O2 takes place at less negative potential with increased peak current attitude at GC/r-GO-mediator. The developed sensor GC/r-GO-mediator, was applied for the continuous monitoring of H2O2 by chronoamperometry. The applied potential was −0.3 V which results in the enhanced sensitivity of the developed biosensor. The developed biosensor shows good stability, and reproducibility. The reproducibility of the developed electrode was evaluated by calculation of relative standard deviation value 6.77%. The linear range was found to be 3.4 – 23.48 µg/ml. Furthermore, limit of detection and limit of quantification were 0.68 µg/ml and 2.27 µg/ml respectively.

Keywords: Nanocomposite, reduced graphene oxide-cerium dioxide, biosensor, biomimetic, chronoamperometry
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