GREEN SYNTHESIS OF SILVER NANOPARTICLES USING SAGE AND THEIR ANTIBACTERIAL EFFECT ON GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA

1,2,3 SEHNAL Karel
Co-authors:
1,2 UHLIROVA Dagmar 1,2 STANKOVA Martina 1,2 VSETICKOVA Michaela 1,2 TOTHOVA Zuzana 1,2,5 HOSNEDLOVA Bozena 6 KEPINSKA Marta 1,3 RUTTKAY-NEDECKÝ Branislav 4 BACH Duong Ngonc 1 SOCHOR Jiri 6 MILNEROWICZ Halina 4 NGUYEN Hoai Viet 1,2,3,5,6 KIZEK Rene
Institutions:
1 Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic, EU, karelsehnal15@gmail.com
2 Department of Research and Development, Prevention Medicals s.r.o., Tovarni 342, 742 13; Studenka-Butovice, Czech Republic, uhlirova@preventionmedicals.cz
3 Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho trida 1946/1, 612 42 Brno, Czech Republic, kizek@sci.muni.cz
4 Research Center for Environmental Monitoring and Modeling, VNU University of Science, 332 Nguyen Trai street, Thanh Xuan district, Hanoi, Vietnam, nguyenviethoai@hus.edu.vn
5 Department of Food and Feed Safety, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
6 Department of Biomedical and Environmental Analyses, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland, zalewska.m@gmail.com
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
469-473
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
79 views / 16 downloads
Abstract

In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green synthesis, plant extracts are used as reducing agents. AgNPs contain surface-bound biomolecules from the used plant extract. We used an aqueous extract from sage (Salvia officinalis). This plant is known for its high content of secondary metabolites with excellent antibacterial activity. Antibacterial activity of AgNPs prepared by green synthesis was investigated. Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were selected for testing. Methods such as agar diffusion assay and inhibition of bacterial growth curves were used. Minimum inhibitory concentrations (MICE. coli = 250 µg/mL; MICS. aureus = 50 µg/mL) were determined. The radiuses of inhibition zones were about 1.5 cm in both bacterial strains. AgNPs prepared by this method can play an important role in dealing with the problem of bacterial resistance. Bacteria develop resistance not only to typical antibiotics. Bacterial cells are also able to eliminate the effect of AgNPs. By modifying their surface with antibacterial molecules, resistance can be effectively combatted.

Keywords: Green synthesis, silver nanoparticles, antibacterial activity, minimum inhibitory concentration
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