COLORIMETRIC AND VISUAL EVALUATION OF SARCOSINE IN THE ARTIFICIAL URINE

1 TOTHOVA Zuzana
Co-authors:
1 UHLIROVA Dagmar 1 STANKOVA Martina 1 VSETICKOVA Michaela 2 RUTTKAY-NEDECKY Branislav 3 KEPINSKA Marta 1 RUZICKA Josef 3 MILNEROWICZ Halina 4 EIAMPHUNGPORN Warawan 1,2,3 KIZEK Rene
Institutions:
1 Department of Research and Development, Prevention Medicals s.r.o., Tovární 342, 742 13; Studénka-Butovice, Czech Republic, uhlirova@preventionmedicals.cz
2 Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1946/1, 612 42 Brno, Czech Republic, kizek@sci.muni.cz
3 Department of Biomedical and Environmental Analyses, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland, zalewska.m@gmail.com
4 Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
474-478
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
48 views / 12 downloads
Abstract

Suitable molecular or biochemical markers are sought for rapid diagnostics of cancer. A very promising candidate is the sarcosine (SAR) amino acid, which is elevated in the urine of the patients with prostate cancer. The aim of this work was to develop a simple, fast and reliable method for SAR detection in human urine. Sarcosine oxidase hydrolyses sarcosine and the resulting peroxide is converted to a colored product. By colorimetric analysis, sarcosine could be detected at low concentrations, limit of detection (LOD) was 1 µM and limit of quantification (LOQ) was 6 µM in artificial urine. As a quick test for the presence of sarcosine in urine, a visual assessment of color change has been proposed. Visual results were evaluated as RGB signal densities. The obtained results show that the average RGB variability between test samples was about 10%. From these values, the ROC curve of the individual experiments was determined. Sensitivity (from 0.59 to 0.83) and specificity (1.00) were calculated using ROC curves according to the type of artificial urine. ROC curves were more than 25% rated as excellent, 65% as very good from 5% as good and none as unsatisfactory. Conclusion: Colorimetric detection of sarcosine in urine was sensitive. Visual evaluation of the test showed 100% success in identifying the presence of sarcosine in artificial urine. The obtained data show the potential of the method for the visual evaluation of the presence of sarcosine in the urine and its possible use in the diagnostics of prostate cancer.

Keywords: Sarcosine, malignant prostate tumors, biosensor, colorimetric detection, visual test
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