PSEUDOPEROXIDASE ACTIVITY OF SILVER NANOPARTICLES (AGNPS) AND CHANGES IN PEROXIDASE ACTIVITY IN MAIZE PLANTS EXPOSED TO AGNPS

1 TOTHOVA Zuzana
Co-authors:
1,2,3 SEHNAL Karel 1,2 UHLIROVA Dagmar 1,2 STANKOVA Martina 1,2 VSETICKOVA Michaela 2 HOSNEDLOVA Bozena 4 KEPINSKA Marta 2,3 RUTTKAY-NEDECKY Branislav 1 RUZICKA Josef 1,3,5 OZDOGAN Yagmur 6 FERNANDEZ Carlos 4 MILNEROWICZ Halina 2 SOCHOR Jiri 1,2,3,4 KIZEK Rene
Institutions:
1 Department of Research and Development, Prevention Medicals s.r.o., Tovarni 342, 742 13; Studenka-Butovice, Czech Republic, EU, uhlirova@preventionmedicals.cz
2 Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic, EU, bozena.hosnedlova@post.cz
3 Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho trida 1946/1, 612 42 Brno, Czech Republic, EU, kizek@sci.muni.cz
4 Department of Biomedical and Environmental Analyses, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland, zalewska.m@gmail.com
5 School of Pharmacy, Altinbas University, Faculty of Pharmacy, Mahmutbey Mahallesi, Dilmenler Caddesi Mahmutbey Yerleskesi 26, 34218 Bagcılar/Istanbul, Turkey
6 School of Pharmacy and Life Sciences, Robert Gordon University, Garthdee Road, Aberdeen, AB10 7QB, Scotland, United Kingdom, c.fernandez@rgu.ac.uk
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
342-347
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
44 views / 8 downloads
Abstract

Some types of nanoparticles such as gold and silver show interesting chemical properties. Pseudoperoxidase activity has been found in iron- or gold-based materials, but some silver nanoparticles (AgNPs) also exhibit such properties. Pseudoperoxidase activity of AgNPs prepared by green synthesis using sage leaves was studied. The AgNPs (prepared by green synthesis using sage leaves) size ranged from 20 to 50 nm and zeta potential was found to vary from -10 to 20 mV, with absorption maximum being 430 nm. The peroxidase-like activity was monitored in the presence of 5 mM 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (30%). The nanoparticles showed about 80% activity of 1 mU horseradish peroxidase (HRP). In addition, AgNPs were applied to maize seedlings (5 days in hydroponics system) at concentrations of 1 mg/L, 50 mg/L, and 150 mg/L. Peroxidase activity in leaves after 48 hours was 59.5, 67.5, and 57.2 µg.mL-1 of HRP, respectively. Peroxidase activity of untreated plant leaves was 58.8 µg.mL-1 of HRP. Peroxidase activity in roots after 48 hours was 101.6, 109.0, and 156.7 µg.mL-1 of HRP, respectively. The peroxidase activity level of the control variant in root of treatment was 98.7 µg.mL-1 of HRP. The results indicate that the determination of peroxidase activity could be used to assess the phytotoxicity of nanoparticles in plants.

Keywords: green synthesis, toxicity of nanoparticles, enzymatic activity, maize, nanotoxicity
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