ISOLATION OF BRANCHED CHAIN AMINOACIDS (VALINE, LEUCINE AND ARGININE) USING MAGHEMITE PARTICLES

1,2 CERNEI Natalia
Co-authors:
1,2 KOPEL Pavel 3 HORKY Pavel 1,2 HYNEK David 1,2 ZITKA Ondrej 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU, vojtech.adam@mendelu.cz
2 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic, EU
3 Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
374-378
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
96 views / 20 downloads
Abstract

In this paper, we report the optimal conditions for the isolation and preconcentration of branched chain amino acids (BCAAs) – leucine, valine and arginine from different types of matrix, such as tissue, plasma, blood, bacteria or cells. Herein, we focused on the synthesis of paramagnetic particles able to isolate and immobilize amino acids and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified nanomaghemite (γ-Fe2O3) by multi walled carbon nanotubes (MWCNT) and hyaluronic acid as the functional carriers providing excellent affinity properties. Our paramagnetic particles have potential for better isolation of BCAAs mainly from plasma or bacteria and in the future they can also be applied as a platform of delivery system.

Keywords: Ion Exchange Chromatography, Maghemite, MWCNT, BCAAs, valine, leucine, arginine
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