COMPARISON OF MICROBIAL INTERACTIONS OF ZINC OXIDE NANOMATERIALS IN VARIOUS SIZE AND SHAPE

1 RUTHERFORD David
Co-authors:
1 JÍRA Jaroslav 2 MIČOVÁ Júlia 3 REMEŠ Zdenek 4 Hsu Hua Shu 1 REZEK Bohuslav
Institutions:
1 Faculty of Electrical Engineering, CVUT, Prague, Czech Republic, david.rutherford@fel.cvut.cz
2 Institute of Chemistry, Institute of Chemistry SAS, Bratislava, Slovakia
3 Institute of Physics, CAS, Prague, Czech Republic
4 Department of Applied Physics, National Ping Tung University, Ping Tung, Taiwan
Conference:
11th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 16th - 18th 2019
Proceedings:
Proceedings 11th International Conference on Nanomaterials - Research & Application
Pages:
330-335
ISBN:
978-80-87294-95-6
ISSN:
2694-930X
Published:
1st April 2020
Proceedings of the conference have been sent to Web of Science and Scopus for evaluation and potential indexing.
Metrics:
39 views / 10 downloads
Abstract

Zinc oxide nanoparticles (ZnO NP) have shown great potential as a novel antibacterial material at a time when resistance towards conventional antibiotics is becoming more prevalent. We report bacteria inactivation by ZnO NP with novel hedgehog-like morphology using model gram-negative (E. coli) and gram-positive (S. aureus) bacteria. E. coli exposed to the novel ZnO hedgehog NP during growth resulted in 4 orders of magnitude reduction in viable cell concentration after 24 h, which is more than 2 orders higher reduction compared to commercially available ZnO NPs with nominal sizes from 50 nm to 20 um. There was a positive correlation between hedgehog NP concentration and bacteria cell concentration reduction within the range tested 0.1 – 1.0 mg/mL. S. aureus was less sensitive to ZnO NP exposure and inactivation effect of various ZnO NP, was comparable. The effect can be thus attributed to direct mechanical damage of the bacterial mebrane that is the most effective for the novel hedgehog ZnO NP. This conclusion was corroborated also by disk diffusion assays.

Keywords: nanotechnology, microbiology, zinc oxide
Scroll to Top