WHOLE-GENOME EXPRESSION ANALYSIS IN THP-1 MACROPHAGE-LIKE CELLS EXPOSED TO DIVERSE NANOMATERIALS

1,2 BRZICOVÁ Táňa
Co-authors:
1 LÍBALOVÁ Helena 1 VRBOVÁ Kristýna 1 SIKOROVÁ Jitka 3 PHILIMONENKO Vlada 4 KLÉMA Jiří 1 TOPINKA Jan 1 RÖSSNER Pavel
Institutions:
1 Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20 Prague, Czech Republic, EU
2 Laboratory for Risk Research and Management, Faculty of Safety Engineering, VSB – Technical University of Ostrava, 700 30 Ostrava, Czech Republic, EU
3 Microscopy Centre, Institute of Molecular Genetics, Czech Academy of Sciences, 142 20 Prague, Czech Republic, EU
4 Department of Cybernetics, Faculty of Electrical Engineering, Czech Technical University in Prague, 121 35 Prague, Czech Republic, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
679-684
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
58 views / 15 downloads
Abstract

From the perspective of the immune system, nanomaterials (NMs) represent invading agents. Macrophages are immune cells residing in all organs and tissues as the first line of defense. Interactions of macrophages with NMs can determine the fate of NMs as well as their potential toxic effects. In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)], towards THP-1 macrophage-like cells. Cells were incubated with non-cytotoxic concentrations (1-25 µg/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. Gene expression profiling revealed a substantially different molecular response following exposure to diverse NMs. While NM-100 did not exert any significant effect on gene expression profile, all other NMs triggered a pro-inflammatory response characterized by an activation of the NF-κB transcription factor and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative and replication stress as well as cell cycle progression and proteasome function. We suppose that genotoxicity of ZnO and Ag NMs leading to DNA damage and alternatively to apoptosis in THP-1 macrophages is probably caused by the extensive intracellular dissolution of these NPs, as confirmed by TEM imaging.

Keywords: Nanomaterials, toxicity, THP-1 macrophages, gene expression profiling

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