GENOTOXICITY OF NANOMATERIALS IN BEAS-2B CELLS ANALYZED BY THE IN VITRO MICRONUCLEUS ASSAY

1 RÖSSNEROVÁ Andrea
Co-authors:
1 ČERVENÁ Tereza 1,2 BRZICOVÁ Táňa 1,3 VRBOVÁ Kristýna 1 SIKOROVÁ Jitka 1,2 TOPINKA Jan 1 RÖSSNER Pavel Jr.
Institutions:
1 Institute of Experimental Medicine, Czech Academy of Sciences, Prague 4, Czech Republic, EU
2 Charles University in Prague, Prague 2, Czech Republic, EU
3 VSB-Technical University of Ostrava, Ostrava, Czech Republic, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
727-732
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
32 views / 9 downloads
Abstract

The tremendous increase of the use of nanomaterials (NMs) has been witnessed during the last decade in many areas of human life including the chemical industry, cosmetics, biomedicine or food technology. The variety of NMs, their unique properties, almost ubiquitous presence and the size range of 1-100 nm raised the interest of toxicologists. The evaluation of the frequency of micronuclei (MN) as a result of the genotoxic events is a broadly utilized and well-established approach in in vitro studies for testing the risk of chemical exposure. Nevertheless, properties of the NMs give rise to the questions concerning the optimal methodological variants of the MN assay.In our study, five types of well-characterized NMs (TiO2: NM-101 and NM-103; SiO2: NM-200; Ag: NM-300K and NM-302) of specific size, shape, or e.g. dimensions of aggregates were involved in the genotoxicity testing using four variants of protocols differing in the time of NM exposure, application of cytochalasin-B combined with simultaneous and delayed co-treatment with nanoparticles (NPs). Bronchial epithelial cells (BEAS-2B) were used in this study to fulfil these tasks. Presence of NPs was controlled by transmission electron microscopy (TEM).Obtained results showed the different genotoxic potential of the various TiO2 and Ag NMs (NM-101<NM-103 and NM-300K>NM-302, respectively). Comparison of all testing strategies revealed, that the level of DNA damage can differ based on the time of exposure and the methodological approach. In general, using cytochalasin-B led most frequently to the increase of the genotoxic potential of the tested NMs.

Keywords: BEAS-2B cells, genotoxicity, micronucleus assay, nanomaterials
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