REGISTRATION OF TULAREMIA BY SPR TECHNIQUE

1 ISAKOVA Alexandra
Co-authors:
2 ZHARNIKOVA Tatyana 2 ZHARNIKOVA Irina 1,3 INDENBOM Andrey
Institutions:
1 A.N. Frumkin Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences, 31 Leninsky prospect, Moscow GSP-1, 119071 Russia, Isakova_Aleks@list.ru, a.indenbom@gmail.com
2 The Federal Government Health Institution “Stavropol Institute For Plague Control" Of The Federal Service For Surveillance In The Sphere Of Protection Of The Consumers And Human Welfare,13–15, Sovetskaya St., Stavropol, 355035, Russia
3 Moscow Institute of Physics and Technology (State University), 9 Institutskiy per., Dolgoprudny, Moscow Region, 141701, Russian Federation
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
567-572
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
18 views / 5 downloads
Abstract

Adsorption of antibodies to tularemia Francisella tularensis (F. name of the genus) on the surface of gold-plated glass chips and the subsequent reaction of antigen-antibody were studied by surface plasmon resonance (SPR) technique. It was found that these antibodies tend to polylayer adsorption on the gold surface. However, washing with a buffer solution leads to desorption of weakly associated molecules, and the monomolecular coating with the same parameters remains on the surface for all initial concentrations of antibody in the solution. The antibody monolayer characterized by surface protein concentration near 2.6 ng/mm3. This value corresponds to the area of about 100 nm2 per molecule of the immunoglobulin. This first adsorption layer adjacent to the surface irreversibly binds to it, which makes it possible to use such a system for subsequent diagnosis of tularemia microbes in solution. It was shown that rapid determination of the microbe in solution up to concentrations of 1.0 × 102 mcl / ml can be carried out using such biosensors using the SPR method. Based on the estimated area per molecule of the adsorbed antibody in the monolayer and the comparatively low signals of the antigen-antibody reaction, a conclusion was made about the lateral arrangement of the antibody molecules in the adsorption layer. The sensitivity of the proposed method can be improved by creating an oriented layer of antibody molecules, which promotes more effective interaction with the antigen.

Keywords: Biosensors, SPR, orientation of protein, antibodies, the adsorption of proteins, Francisella tularensis (F. tularensis)
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