The present work is focused on preparation of chitosan films, which were doped with silver nanoparticles (AgNPs). AgNPs were prepared and stabilized by reduction of silver nitrate by chitosan without addition of other reducing agents. It was tested whether the use of UV light could affect the AgNPs in the process. The UV light irradiation took 5 min: during the preparation of chitosan solution and at the end of the process when the chitosan films were done. The presence of AgNPs was studied by X-ray photoelectron spectroscopy. Wettability and water absorption of the films were evaluated. Solid films were dissolved and then the solution was observed by the ultraviolet–visible spectroscopy and transmission electron microscopy. Concentration of the AgNPs released into the solution during dissolution was studied by atomic absorption spectroscopy. The presence of AgNPs was confirmed both in the solid films and in the solutions by the above mentioned methods. Our research was aiming on use of these films in medicine as a new type of wound dressing with antibacterial properties. These films could be used as a wound dressing, antimicrobial packaging material or as a long-term storage of AgNPs for various applications.Keywords: Polysaccharides films, silver nanoparticles, chitosan, film preparation and characterization
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