INFLUENCE OF LIPOSOMAL PLATINUM CYTOSTATICS ON CANCER CELLS BY VOLTAMMETRIC METHODS

1,3 SEDLACKOVA Eliska
Co-authors:
2 LANIKOVA Petra 1,3 HYNEK David 1 KREJCOVA Lida 1 BUCHTELOVA Hana 1,3 RICHTERA Lukas 1,3 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2 Department of Biomedical Engineering, Brno University of Technology, Technicka 12, CZ-616 00 Brno, Czech Republic
3 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
548-553
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
11 views / 4 downloads
Abstract

Aim of this work deals with closing of cisplatin into liposomes, the study of their stability and interaction with cancer cell lines. Commercial cisplatin cytostatic was encapsulated into three types of liposomes (L8, L10 and L15) of a different chemical composition. Each of liposomes had different ability to close cytostatic. L8 differs from L10 by containing cholesterol. In despite of L10 and L8, L15 liposome contains a synthetic 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (C41H78NO8P) – DOPE, which is generally found in cell membranes. The size of the liposomes was characterized by Dynamic Light Scattering (DLS). The amount of Pt-drug in liposomes was verified by electrochemical determination. Their stability was observed by differential pulse voltammetry (DPV). These studies also demonstrated, that the cisplatin is stable in physiological solution for at least 24 hours, which making it a suitable medium storage for this drug. Finally, the encapsulated cisplatin was applied to cancer cell lines and their viability was observed by MTT assay.

Keywords: Cisplatin, liposomes, electrochemistry, differential pulse voltammetry, cancer cell lines,
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