BIOPHYSICAL STUDY OF CDTE QUANTUM DOTS INTERACTIONS WITH ALBUMIN AND ANTIBODY AS THE BASE OF PHOTODYNAMIC THERAPY

1 RUTTKAY-NEDECKY Branislav
Co-authors:
1,2 SOCHOROVA Aneta 1,3 DOCEKALOVA Michaela 1,3 UHLIROVA Dagmar 1,3 STANKOVA Martina 4 KEPINSKA Marta 1 SKALICKOVA Sylvie 1,2 CIHALOVA Kristyna 4 MILNEROWICZ Halina 5 FERNANDEZ Carlos 6 ZIDKOVA Jarmila 1,3,4 KIZEK Rene
Institutions:
1 University of Veterinary and Pharmaceutical Sciences Brno, Pharmaceutical Faculty, Brno, Czech Republic, EU
2 Secondary School of Dairy Industry and Higher Vocational School of Food Technology, Kromeriz, Czech Republic, EU
3 Prevention Medicals, Studenka, Czech Republic, EU
4 Wroclaw Medical University, Borowska 211, 50-556, Wroclaw, Poland, EU
5 Robert Gordon University, School of Pharmacy and Life Sciences, Aberdeen, United Kingdom
6 University of Chemistry and Technology Prague, Faculty of Food and Biochemical Technology, Prague 166 28, Czech Republic, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
537-542
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
12 views / 9 downloads
Abstract

Photodynamic therapy is a relatively new type of cancer treatment, or eventually it is used at the elimination of undesirable pathogenic microorganisms. Quantum dots (QDs) are semiconducting nanocrystals with a size of 2-20 nm and can be used in photodynamic therapy. Two types of CdTe QDs were prepared by microwave synthesis (500 W), typical absorption spectra had maxima λgreen = 554 nm, λ yellow = 580 nm. The determined size of the generated nanoparticles ranged between 5 - 10 nm. CdTe QDs were further studied by fluorescence analysis at excitation wavelength λ = 250 nm to obtain emission maxima (λem552) for green and (λem582) for yellow nanoparticles. Interaction study of CdTe QDs with bovine serum albumin (BSA) and with polyclonal chicken antibodies against sarcosine (AntiSar) was performed. BSA (100, 50, 25, 12.5, 6.3, 3.1, 1.6 and 0 μM) and AntiSar (20, 10, 5, 2.5, 1.3, 0.7, 0.3, and 0 g/L) were monitored with 1:1 addition of 50 μM QDsgreen or QDsyellow. The decrease in intensity of the normalized fluorescence signal in the presence of BSA or AntiSar was observed by 70-90%. The observed dependencies showed a linear trend (R2 = 0.9) with relative error of 9-12% calculated from 5 independent repetitions. In addition, it was possible to monitor the signal shift to shorter wavelengths at the highest applied BSA concentration by 4-16 nm. The obtained data suggest that the size of individual QDs will affect intensity of an interaction with biomolecule. Nanoconstructs should therefore be targeted according to these experimental data for their intended use. In further experiments, CdTe QDs modified with AntiSar will be used for targeted prostate cancer therapy using photodynamic effect.

Keywords: Reactive oxygen species, prostate cancer, photodynamic therapy, CdTe quantum dots, nanomedicine
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