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Cisplatin (cis-diamminedichloroplatinum II) is cytostatic currently used in the treatment of a various type of solid tumors, including cancers of the ovary, testis, bladder, head and neck, lung, cervix and endometrium. The health effects of platinum are strongly dependent upon the kind of bonds that are shaped and the exposure level and immunity of the person that is exposed. An in vivo interaction study was performed as a comparison of amino acid profiles in chicken embryo tissue before and after application of cisplatin and two platinum nanoparticles covered with polyvinylpyrrolidone. We found that cisplatin directly influences kidney amino acid representation even at low concentrations. In addition, we performed an interaction study that resulted in the determination of breaking points for each of analyzed amino acids. Proline, leucine, valine, serine, glutamine and isoleucine were determined as the most sensitive amino acids. Additionally, we compared amino acid profiles of liver and kidney before and after exposure to cisplatin and platinum nanoparticles. The amount of amino acids after interaction with cisplatin and platinum nanoparticles was significantly reduced (p ˂ 0.05). This fact points at an ability of cisplatin and Pt nanoparticles to induce changes in quantitative composition of amino acids in chicken tissues. Moreover, this confirms that the interactions between cisplatin, platinum nanoparticles and amino acids may act as another factor most likely responsible for adverse effects of cisplatin and platinum nanoparticles on chicken embryo tissues.
Keywords: Kidney, liver, toxicity, amino acids, ion-exchange liquid chromatography, platinum nanoparticles© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.