INFLUENCE OF ANTINEOPLASTIC DRUG CISPLATIN AND PLATINUM NANOPARTICLES ON CHICKEN EMBRYO LIVER AND KIDNEY AMINO ACIDS

1,2 MILOSAVLJEVIC Vedran
Co-authors:
1,2 CERNEI Natalia 1,2 BYTESNIKOVA Zuzana 1,2 KOPEL Pavel
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
511-516
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
11 views / 3 downloads
Abstract

Cisplatin (cis-diamminedichloroplatinum II) is cytostatic currently used in the treatment of a various type of solid tumors, including cancers of the ovary, testis, bladder, head and neck, lung, cervix and endometrium. The health effects of platinum are strongly dependent upon the kind of bonds that are shaped and the exposure level and immunity of the person that is exposed. An in vivo interaction study was performed as a comparison of amino acid profiles in chicken embryo tissue before and after application of cisplatin and two platinum nanoparticles covered with polyvinylpyrrolidone. We found that cisplatin directly influences kidney amino acid representation even at low concentrations. In addition, we performed an interaction study that resulted in the determination of breaking points for each of analyzed amino acids. Proline, leucine, valine, serine, glutamine and isoleucine were determined as the most sensitive amino acids. Additionally, we compared amino acid profiles of liver and kidney before and after exposure to cisplatin and platinum nanoparticles. The amount of amino acids after interaction with cisplatin and platinum nanoparticles was significantly reduced (p ˂ 0.05). This fact points at an ability of cisplatin and Pt nanoparticles to induce changes in quantitative composition of amino acids in chicken tissues. Moreover, this confirms that the interactions between cisplatin, platinum nanoparticles and amino acids may act as another factor most likely responsible for adverse effects of cisplatin and platinum nanoparticles on chicken embryo tissues.

Keywords: Kidney, liver, toxicity, amino acids, ion-exchange liquid chromatography, platinum nanoparticles
Scroll to Top