INTERACTION OF PLATINUM-BASED CYTOSTATICS AND PLATINUM NANOPARTICLES WITH METALLOTHIONEIN - POTENCIAL SOURCE OF THE ANTITUMOR DRUG RESISTENCE

1 ZELNÍČKOVÁ Jaroslava
Co-authors:
1,2 NEJDL Lukáš 1,2 RICHTERA Lukáš 1,2 KOPEL Pavel 1,2 ADAM Vojtěch
Institutions:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, CZ-613 00 Brno, Czech Republic
2 SIX Centre, Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
505-510
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
11 views / 3 downloads
Abstract

Platinum-based cytostatic represent a unique class of DNA-damaging antitumor agents and they are one the most frequently used drugs in oncology. Problem is that some kind of cancer is resistant against this type of cytostatics. This resistance can be potentially caused by metalloproteins such as metallothioneins. MTs belong to the group of intracellular cystine-rich, metal-binding proteins and hold a number of functions in body. One of them is detoxification of heavy metals. This ability of MTs can cause a decreased therapeutic effect of platinum-based cytostatics. In this work, the interaction between two isoforms of MTs (MT3 and MT2a) and several types of platinum cytostatics (oxaliplatin, carboplatin and cisplatin) as well as platinum nanoparticles (size of 10 and 40 nm) was examined by fluorimetric analysis using a fluorescence zinc indicator (Fluozin-3). Both, stationary fluorescence spectrometry and capillary electrophoresis with laser-induced fluorescence detection (ex – 488 nm, em – 530 nm) was used in the study.

Keywords: Platinum, nanoparticle, cytostatics, fluorescence, metalloprotein
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