OPTICAL PROPERTIES OF DETONATION NANODIAMOND COMPLEXES WITH GLOBULAR PROTEINS IN WATER SOLUTION

1,2 BUTUSOV Leonid
Co-authors:
2,3,4 NAGOVITSYN Ilia 1 SHULGA Aleksandra 2,4 CHUDINOVA Galina 2,4 VLASOV Igor 1 HAIRULLINA Indira 1 KURILKIN Vladimir 1 KOCHNEVA Margarita
Institutions:
1 RUDN University (People’s Friendship University of Russia), Moscow, Russian Federation
2 Natural Science Center of General Physics Institute RAS, Moscow, Russian Federation
3 Semenov Institute of Chemical Physics RAS, Moscow, Russian Federation
4 National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russian Federation
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
495-498
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
14 views / 9 downloads
Abstract

It has been studied the changing of spectral characteristics of water-soluble 10 nm detonation nanodiamonds (DND) upon their complexation with a globular blood protein: bovine hemoglobin (Hb), human serum albumin (HSA) and rabbit immunoglobulin G (IgG) by fluorescence and absorption spectroscopy. The structure of the protein globule and its concentration in aqueous solution can significantly affect the complexation with DND (2·10-3 g/l) in the process of ultrasonic treatment (42 kHz). In all cases, it was observed when adding the DND the increasing of intensity of own luminescence of proteins (λem = 330-346 nm) and bathochromic shift of protein luminescence bands. The magnitude of the shift of the maximum of protein luminescence bands at a protein concentration of 10-11 M was 5, 16, 18 nm, at the concentration 10-5 M - 7, 9, 2 nm for Hb, HSA and IgG, respectively. The aggregates’ size of DND was evaluated by AFM method in monocomponent film on the glass substrate. The results are of interest for investigations of the dynamic interaction of DND with biomacromolecules in vivo.

Keywords: Detonation nanodiamonds, hemoglobin, albumin, immunoglobulin G, luminescence
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