HUMAN STEM CELL-DERIVED TISSUE ORGANOIDS AS A POTENTIAL TOOL FOR DRUG DELIVERY TESTING

1 JOSE Shyam Sushama
Co-authors:
1 TIDU Federico 1 FRIČ Jan
Institution:
1 Cellular and Molecular Immunoregulation Group, Center for Translational Medicine, International Clinical Research Center, St. Anne's University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech Republic, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
589-592
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
12 views / 8 downloads
Abstract

Advancement in organoid cultures from human pluripotent stem cells allows studying complexity of tissue specific interactions in vitro. This organ in a chip approach provides an important tool for translational research. Successful development of nanoparticles and nanostructures for clinical use has increased the need for more reliable and high-throughput experimental models beyond conventional cell culture. Human induced pluripotent stem cells (iPSCs) can be differentiated to multiple cell types including tissue-like organoids. Here, we study the interaction of nanoparticles with differentiated tissue cells and analyse their effects on various organoids in vitro. Human tissue organoids are differentiated from iPSCs by growth factor and by manipulation of various developmental signal pathways with inhibitors. The 3-dimentional structures of the bodies, with structural features similar to the appropriate human organ/tissue are obtained by embedding in a matrix. In order to test organoid as a model for interaction with nanoparticles, the organoids are injected with nano-sized particulate triggers such as glucans. The various tissue models are compared for their nanoparticle-induced stimulation by screening for inflammatory cytokines. Described model will allow studying human tissue interaction with nanoparticles, which for example are used in drug delivery.

Keywords: Nanoparticles, organ in a chip, lung-organoid, stem cells, lipopolysaccharide
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