NANOPARTICLES OF ZNO - CHANGE OF ZETA POTENTIAL AND ADSORPTION OF BSA

1 BUKÁČKOVÁ Marta
Co-authors:
1 MARŠÁLEK Roman
Institution:
1 University of Ostrava, Ostrava, Czech Republic, EU, marta.bukackova@osu.cz
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
473-478
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
13 views / 2 downloads
Abstract

This study was focused on interaction ZnO nanoparticles with protein. Bovine Serum Albumin (BSA) was chosen as a model protein to show how successful is the adsorption of protein on our prepared nanoparticles. Particles of ZnO were prepared by direct precipitation method from Zn(NO3)2 by addition of urea and further treatment. These particles were dispersed in water and characterized by distribution of size and compared with commercial ZnO. Average size of dispersed prepared particles was around 75 nm. Afterwards the zeta potential of particles was measured in different conditions (change of pH, concentration, ionic strength) and the isoelectric point was determined. These results were used for optimization the appropriate conditions for adsorption of BSA on ZnO nanoparticles. The changes of zeta potential were measured during adsorption of BSA on ZnO nanoparticles. These results can help to understand the interaction of ZnO nanoparticles with protein which is important to know for use their antibacterial effect in application in medicine.

Keywords: zinc oxide, bovine serum albumin, zeta potential, adsorption
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