This study was focused on interaction ZnO nanoparticles with protein. Bovine Serum Albumin (BSA) was chosen as a model protein to show how successful is the adsorption of protein on our prepared nanoparticles. Particles of ZnO were prepared by direct precipitation method from Zn(NO3)2 by addition of urea and further treatment. These particles were dispersed in water and characterized by distribution of size and compared with commercial ZnO. Average size of dispersed prepared particles was around 75 nm. Afterwards the zeta potential of particles was measured in different conditions (change of pH, concentration, ionic strength) and the isoelectric point was determined. These results were used for optimization the appropriate conditions for adsorption of BSA on ZnO nanoparticles. The changes of zeta potential were measured during adsorption of BSA on ZnO nanoparticles. These results can help to understand the interaction of ZnO nanoparticles with protein which is important to know for use their antibacterial effect in application in medicine.Keywords: zinc oxide, bovine serum albumin, zeta potential, adsorption
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.