TRACKING TRANSCRIPTION FACTOR ACTIVITY IN 3D NANOSCAFFOLD

1,2 Tidu F.
Co-authors:
3,4 Bachmann B. 1 Bendickova K. 1,2 Jose S. S. 4 Ertl P. 1 Fric J.
Institutions:
1 Cellular and Molecular Immunoregulation Group, Center for Translational Medicine, International Clinical Research Center, St. Anne's University Hospital Brno, Czech Republic, EU
2 Department of Biology, Faculty of Medicine, Masaryk University, Czech Republic, EU
3 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Wien, Austria, EU
4 TU Wien: Institute of Applied Synthetic Chemistry, Vienna University of Technology, Wien, Austria, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
578-581
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
11 views / 2 downloads
Abstract

Mesenchymal stem cells (MSCs) are multipotent cells present in the stromal fraction of many tissues. Their immunomodulatory properties and differentiation potential have been proven beneficial in number of autoimmune disease therapy models. However a reliable in vitro approach to study MSCs differentiation in inflamed tissue and a clear insight into the involved signalling pathways may accelerate their application in vivo.Fibrin nanoscaffolds have been developed for cartilage tissue engineering and provide a natural matrix that can mimic synovium microenvironment. Moreover fibrin hydrogels allow recreating in vitro the same stiffness chondrocytes face in vivo, leading to improved chondrogenesis differentiation. Besides all these properties, fibrin can be completely degraded by nattokinase, allowing an easy single cell analysis.The aim of this work is to investigate the role of NF-kB inflammatory pathway in MSCs chondrogenesis performed within fibrin nanoscaffolds. Thus we established NF-kB luciferase reporter line to monitor transcription factor activation. Combining degradable fibrin nanoscaffold technology to reporter line for monitoring transcription factors activity will provide a powerful platform to understand the role of inflammation in different stages of chondrogenesis.

Keywords: Nanoscaffold, fibrin hydrogel, mesenchymal stem cells, inflammation
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