In our study, we aimed to synthesize superparamagnetic nanoparticles (PMPs) able to isolate and immobilize and thus preconcentrate flavonoids for subsequent analysis. We report the optimal conditions such as different pH of Britton Robinson buffer, temperature and time of incubation on the isolation of different types of flavonoids, such as Genkwanin and Pratol from extracts of freshwater algae. For the determination and their characterization was used an Agilent 1200 Series Rapid Resolution LC system coupled on-line Agilent Technologies 6460 Triple quadruple MS detector with Agilent Jet Stream (all from Agilent Technologies, Waldbronn, Germany). We modified surface of nanomaghemite (γ-Fe2O3) by chitosan and amine groups. One type of PMPs superparamagnetic nanoparticle was able to bind Genkwanin and Pratol with 34.49 % and 27.65 % recoveries, respectively. In future, the nanoparticles can serve for application and as a platform of biosensors.Keywords: Flavonoids; Maghemite nanoparticles; genkwanin; pratol
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