GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZNO NANOPARTICLES

1 ROSSNER Pavel Jr.
Co-authors:
1 VRBOVA Kristyna 2 STRAPACOVA Simona 1 ROSSNEROVA Andrea 1 AMBROZ Antonin 1,3 BRZICOVA Tana 1 LIBALOVA Helena 1 JAVORKOVA Eliska 1 ZAJICOVA Alena 1 HOLAN Vladimir 2 KULICH Pavel 4 VECERA Zbynek 4 MIKUSKA Pavel 4 COUFALIK Pavel 4 KRUMAL Kamil 4 CAPKA Lukas 4 DOCEKAL Bohumil 5 SERY Omar 5 MISEK Ivan 2 MACHALA Miroslav 1 TOPINKA Jan
Institutions:
1 Institute of Experimental Medicine, Czech Academy of Sciences, Prague 4, Czech Republic, EU
2 Veterinary Research Institute, Brno, Czech Republic, EU
3 VSB - Technical University of Ostrava, Ostrava, Czech Republic
4 Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic, EU
5 Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic, EU
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
650-655
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
10 views / 2 downloads
Abstract

We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles (NP). Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression; three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.

Keywords: Zinc oxide nanoparticles, Inhalation, Gene expression, Immune response
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