PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS

1,2 FARKA Zdeněk
Co-authors:
1,2,3 HLAVÁČEK Antonín 2 MICKERT Matthias J. 1 SKLÁDAL Petr 2 GORRIS Hans H.
Institutions:
1 CEITEC – Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic
2 Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany
3 Institute of Analytical Chemistry CAS, v. v. i., 602 00 Brno, Czech Republic
Conference:
9th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 18th - 20th 2017
Proceedings:
Proceedings 9th International Conference on Nanomaterials - Research & Application
Pages:
455-460
ISBN:
978-80-87294-81-9
ISSN:
2694-930X
Published:
8th March 2018
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
25 views / 3 downloads
Abstract

Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg·mL−1.

Keywords: photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac
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