The antibacterial effect of thin films TiO2:SiO2:Ag on two Gram-negative bacteria Escherichia coli and Pseudomonas putida was investigated. The thin films were deposited on glass substrates without heating by r.f. magnetron co-sputtering of TiO2 target with small plates of quartz and Ag on its surface in the zone with maximum erosion. The total area of quartz plates was 750 mm2 and the area of Ag plates was 40 mm2 and 100 mm2. High resistant industrial strain E.coli (ATCC 10536) and sensitive P. Putida (ATCC 12633) with an ability to form biofilm were chosen for the test. Three experimental techniques were used to estimate the antibacterial activity: classical Koch’s method, optical density measurements, and dehydrogenase activity inhibition. The bacteria were cultivated on the studied thin films in a liquid nutrient medium for 24 h without agitation. Complete deactivation of the bacterial growth was observed between the 1st and the 4th hours from the beginning of the experiment. The destruction of the bacterial cells was registered by scanning electron microscope. The developed thin films have a potential for application as antibacterial coating for different medical devices and surfaces.Keywords: TiO2:SiO2:Ag thin films, bactericidal effect, gram-negative bacteria
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