A COMPARISON OF THE CENTRIFUGAL FORCE SPINNING AND ELECTROSPINNING OF COLLAGEN UNDER DIFFERENT CONDITIONS

1 HLAVATÁ Jana
Co-authors:
2,3 SUCHÝ Tomáš 2 ŠUPOVÁ Monika 3 POKORNÝ Marek 1 KUŽELOVÁ KOŠŤÁKOVÁ Eva
Institutions:
1 Technical University of Liberec, Liberec, Czech Republic, EU
2 IRSM AS CR, Department of Composites and Carbon Materials, Prague, Czech Republic, EU
2 CTU in Prague, Faculty of Mechanical Engineering, Prague, Czech Republic, EU
3 Contipro, a.s., R&D Department, Dolní Dobrouč, Czech Republic, EU
Conference:
8th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 19th - 21st 2016
Proceedings:
Proceedings 8th International Conference on Nanomaterials - Research & Application
Pages:
514-519
ISBN:
978-80-87294-71-0
ISSN:
2694-930X
Published:
17th March 2017
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
34 views / 16 downloads
Abstract

Collagen makes up one of the most important parts of the extracellular matrix and connective tissue. Collagen type I accounts for up to 90% of the collagen found in the body and it can be isolated and purified in large quantities and processed into a variety of forms. With respect to the process of the treatment of collagen into the nano-fibrous and submicron-fibrous forms, severe conditions leading to the denaturation of the collagen and thus the gelatin content should be avoided. Centrifugal force spinning and electrospinning processes appear to present promising methods for the spinning of collagen solutions. This paper deals with a comparison of the various methods applied in the processing of collagen, i.e. principally needle and needleless centrifugal spinning technology and electrospinning technology. In addition, the effect of process parameters and collagen solution conditions on the final form of the material was analyzed. Spun layers were prepared based on collagen type I isolated from calf skin. Nano-structured layers were prepared employing the spinning of 4–16 wt% collagen solutions in phosphate buffer saline and ethanol. The layers thus prepared were characterized by means of scanning electron microscopy and Fourier transform infrared spectroscopy.

Keywords: Collagen; electrospinning; centrifugal force spinning; triple-helix
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