SUITABILITY OF LYOPHILIZED POLYMERS BASED ON CMC FOR CELL PROLIFERATION

1 BORŮVKOVÁ Karolína
Co-authors:
1 WIENER Jakub 2 KAMINSKÁ Marta 1 KOLÍNOVÁ Marcela
Institutions:
1 Technical University of Liberec, Studentská 2, Liberec, Czech Republic, EU, karolina.boruvkova@tul.cz, jakub.wiener@tul.cz, marcela.kolinova@tul.cz
2 Lodz University of technology, Institute of Materials Science and Engineering, ul. Stefanowskiego 1/15, Lodz, Poland, EU, marta.kaminska@p.lodz.pl
Conference:
7th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 14th - 16th 2015
Proceedings:
Proceedings 7th International Conference on Nanomaterials - Research & Application
Pages:
565-570
ISBN:
978-80-87294-59-8
ISSN:
2694-930X
Published:
11th January 2016
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
23 views / 3 downloads
Abstract

The aim of the study was to develop a material suitable for wound dressing which would be also suitable for cell growth and cell proliferation. Such matrix should have the structure of interconnected pores and a high porosity to ensure the adequate penetration of the cells and the supply of nutrients to the cells during their formation, diffusion of waste products out of the matrix and exclusion the products of matrix degradation. The study is focused on optimization the method of preparation of 3D porous structures and subsequent testing of cytotoxicity of thus prepared structures. The cytotoxicity of the structers was tested on human skin fibroblast cells. The cytotoxicity was tested by measuring the cell viability and using XTT assay. Another performed test was the test of biocidity/biodegradability. XTT assay results demonstrated that the lyophilized sample of carboxymethylcellulose is suitable for cell proliferation. Better cell proliferation is also caused by the increase of porosity made by lyophilization.

Keywords: lyophilization, carboxymethylcellulose, cells, fibroblasts, toxicity
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