from the conferences organized by TANGER Ltd. provisional website
The aim of the study was to develop a material suitable for wound dressing which would be also suitable for cell growth and cell proliferation. Such matrix should have the structure of interconnected pores and a high porosity to ensure the adequate penetration of the cells and the supply of nutrients to the cells during their formation, diffusion of waste products out of the matrix and exclusion the products of matrix degradation. The study is focused on optimization the method of preparation of 3D porous structures and subsequent testing of cytotoxicity of thus prepared structures. The cytotoxicity of the structers was tested on human skin fibroblast cells. The cytotoxicity was tested by measuring the cell viability and using XTT assay. Another performed test was the test of biocidity/biodegradability. XTT assay results demonstrated that the lyophilized sample of carboxymethylcellulose is suitable for cell proliferation. Better cell proliferation is also caused by the increase of porosity made by lyophilization.Keywords: lyophilization, carboxymethylcellulose, cells, fibroblasts, toxicity
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.