GROWTH AND DIFFERENTIATION OF HUMAN OSTEOBLASTLIKE CELLS ON TI6AL4V ALLOY MODIFIED WITH ANODIZATION

1 DOUBKOVA Martina
Co-authors:
1 BACAKOVA Lucie 1 PARIZEK Martin 1 VANDROVCOVA Marta 1 MUSILKOVA Jana 1 LISA Vera 2 GABOR Roman 3 MARVAN Jaroslav
Institutions:
1 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic, EU, Martina.Doubkova@fgu.cas.cz
2 Laboratories and Testing Rooms VUHZ Joint-Stock Co., Chemical and Radio-Isotopes Laboratory, Dobra, Czech Republic, EU, Gabor@vuhz.cz
3 Medin Joint-Stock Co., Nove Mesto na Morave, Czech Republic, EU, export@medin.cz
Conference:
7th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 14th - 16th 2015
Proceedings:
Proceedings 7th International Conference on Nanomaterials - Research & Application
Pages:
460-465
ISBN:
978-80-87294-59-8
ISSN:
2694-930X
Published:
11th January 2016
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
18 views / 2 downloads
Abstract

Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblastlike SAOS-2 cells on Ti6Al4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Nonanodized Ti6Al4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a nonhomogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.

Keywords: Ti6Al4V, SAOS2 cells, anodization, bone implants, collagen I, mineralization
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