GROWTH OF HUMAN ADIPOSE-DERIVED STEM CELLS ON TI-6AL-4V ALLOY WITH VARIOUS SURFACE MODIFICATIONS

1 KROČILOVÁ Nikola
Co-authors:
1 BAČÁKOVÁ Lucie 1 PAŘÍZEK Martin 1 HAVLÍKOVÁ Jana 2 MOTARJEMI Hooman 2 MOLITOR Martin 3 GABOR Roman 4 MARVAN Jaroslav
Institutions:
1 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
2 Department of Plastic Surgery, Bulovka Hospital in Prague, Czech Republic, n.kroci@seznam.cz
3 Division of Laboratories and Testing, Chemical and Radio-Isotopes Laboratory, VUHZ Joint-Stock Co., Dobra, Czech Republic, EU
4 Medin Joint-Stock Co., Nove Mesto na Morave, Czech Republic, EU
Conference:
7th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 14th - 16th 2015
Proceedings:
Proceedings 7th International Conference on Nanomaterials - Research & Application
Pages:
466-471
ISBN:
978-80-87294-59-8
ISSN:
2694-930X
Published:
11th January 2016
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
45 views / 5 downloads
Abstract

Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter Ra = 280 nm) or modified by shot blasting and tarnishing (A, Ra = 200 nm), vibratory finishing (B, Ra = 100 nm) and vibratory finishing, shot blasting and polishing (C, Ra = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells.

Keywords: bone implants, bone tissue engineering, surface roughness, stem cells, liposuction, cell proliferation

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