FLUORESCENCE STUDY OF MICROVISCOSITY OF HYALURONAN HYDROGELS CONTAINING HYDROPHOBIC NANODOMAINS DURING FOURTEEN DAYS OF AGEING

1 HOLÍNKOVÁ Petra
Co-authors:
1 MRAVEC Filip 1 PEKAŘ Miloslav
Institution:
1 Brno University of Technology, Faculty of Chemistry, Institute of Physical and Applied Chemistry, Purkyňova 464/118, 612 00 Brno, xcucekajova@fch.vutbr.cz
Conference:
7th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 14th - 16th 2015
Proceedings:
Proceedings 7th International Conference on Nanomaterials - Research & Application
Pages:
408-412
ISBN:
978-80-87294-59-8
ISSN:
2694-930X
Published:
11th January 2016
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
436 views / 169 downloads
Abstract

This paper deals with the fluorescence study of microviscosity changes of hydrogel composed by hyaluronan and cationic surfactant cetyltrimethylammonium bromide (CTAB) during fortnight-ageing. The steady state anisotropy values obtained in the experiments were used to estimate the microviscosity of the corresponding hydrogel regions. Two different fluorescent probes were used, perylene for hydrophobic domains and rhodamine 6G for hydrophilic region. It is observed that the microviscosity in the hydrophobic domains of hydrogel is roughly constant, indicating that these domains do not undergo significant structural changes during ageing. However, the microviscosity of hydrophilic parts gradually decreases, which indicates structural changes in the nearby area of rhodamine molecules.

Keywords: fluorescence anisotropy, microviscosity, hyaluronan, CTAB, hydrogels

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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