CONSTANT CURRENT CHRONOPOTENTIOMETRY STUDY OF DNA FOR THE DETECTION OF AFRICAN SWINE FEVER VIRUS

1 Rychly Ondrej
Co-authors:
1,2,5 Hosnedlova Bozena 3 Parikesit Arli Aditya 2 JAKUBEK Milan 4 Kepinska Marta 5 VASICKOVA Petra 1,2,4,5 Kizek Rene
Institutions:
1 CONEM Metallomics Nanomedicine Research Group (CMNRG), Vinohrady, Prague, Czech Republic, EU, rychly@seznam.cz
2 BIOCEV, First Faculty of Medicine, Charles University, Vestec, Czech Republic, EU, bozena.hosnedlova@post.cz; milan.jakubek@lf1.cuni.cz
3 Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta Timur, Indonesia, pakresit@el.ind
4 Department of Pharmaceutical Biochemistry, Division of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland, EU, marta.kepinska@umw.edu.pl
5 Veterinary Research Institute, Brno, Czech Republic, EU, kizek@sci.muni.cz
Conference:
13th International Conference on Nanomaterials - Research & Application, Orea Congress Hotel Brno, Czech Republic, EU, October 20 - 22, 2021
Proceedings:
Proceedings 13th International Conference on Nanomaterials - Research & Application
Pages:
290-297
ISBN:
978-80-88365-00-6
ISSN:
2694-930X
Published:
22nd November 2021
Proceedings of the conference have already been published in Scopus and we are waiting for evaluation and potential indexing in Web of Science.
Metrics:
153 views / 69 downloads
Abstract

African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of the specific nucleic acid of ASFV using electrochemical detection. Determination of DNA was conducted by chronopotentiometric stripping analysis (time of accumulation 120 s, stripping current 20, 10, 5, 1 µA, measurement time 240 s). The volume of the analyzed sample was 10 µL. Sufficient analysis duration (200‒600 s) is required to fully record the H peak signal of DNA at a given applied current. Extending the DNA analysis time at a given current significantly improved the dt/dE records of the H peak and the reproducibility of the analysis (RSD up to 10%). It was found that the suitable time at which the H peak is plotted reliably is around 240 s. The detected dsDNA concentration dependence (0 to 60 µg/mL) on the catalytic signal of the H peak was described according to the equation y = 260.4 + 366.7x, r = 0.961, RSD 23.1%. Subsequently, the SPION particle was modified with dsDNA. After the thermal release of DNA from SPION, DNA was immediately determined by chronopotentiometric stripping analysis method.

Keywords: Emergency biosensor; nucleic acid; oligonucleotide; superparamagnetic nanoparticle

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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