Open Access Conference Proceedings

African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of the specific nucleic acid of ASFV using electrochemical detection. Determination of DNA was conducted by chronopotentiometric stripping analysis (time of accumulation 120 s, stripping current 20, 10, 5, 1 µA, measurement time 240 s). The volume of the analyzed sample was 10 µL. Sufficient analysis duration (200‒600 s) is required to fully record the H peak signal of DNA at a given applied current. Extending the DNA analysis time at a given current significantly improved the dt/dE records of the H peak and the reproducibility of the analysis (RSD up to 10%). It was found that the suitable time at which the H peak is plotted reliably is around 240 s. The detected dsDNA concentration dependence (0 to 60 µg/mL) on the catalytic signal of the H peak was described according to the equation y = 260.4 + 366.7x, r = 0.961, RSD 23.1%. Subsequently, the SPION particle was modified with dsDNA. After the thermal release of DNA from SPION, DNA was immediately determined by chronopotentiometric stripping analysis method.

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