Threads are the basic textile linear structure used in many areas. The variability of the thread structure made possible by special constructions brings great possibilities of variability. It is also possible to introduce nanofiber material into the yarn construction as a cover for central microfiber yarns, and these can be further intertwined into more complex yarn constructions. Continuous production of so-called composite yarns containing central microfiber yarns and nanofiber wrappers is made possible by alternating current electrospinning. Subsequent braiding of these composite yarns allows the protection of nanofiber packages and the variability of the overall construction of the resulting yarns. This paper introduces the creation of yarns and various types of basic as well as braided yarns with nanofibers. Selected sutures were further subjected to in-vitro testing to verify cytocompatibility for application in hygiene such as dental sutures or medicine as surgical sutures.Keywords: AC electrospinning, composite nanofibrous yarns, braiding, cytocompatibility, dental floss
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