SENSITIVE AMPEROMETRIC NANOBIOSENSOR FOR DETECTION OF SARCOSINE - POTENTIAL PROSTATE CANCER MARKER - IN URINE SAMPLES

1 HRONČEKOVÁ Štefánia
Co-authors:
1 BERTÓK Tomáš 1 TKÁČ Ján
Institution:
1 Slovak Academy of Sciences, Institute of chemistry, Bratislava, Slovakia, EU, chemshrn@savba.sk
Conference:
13th International Conference on Nanomaterials - Research & Application, Orea Congress Hotel Brno, Czech Republic, EU, October 20 - 22, 2021
Proceedings:
Proceedings 13th International Conference on Nanomaterials - Research & Application
Pages:
215-220
ISBN:
978-80-88365-00-6
ISSN:
2694-930X
Published:
22nd November 2021
Proceedings of the conference were published in Scopus.
Metrics:
292 views / 182 downloads
Abstract

In this work, two-dimensional layered material Ti3C2TX MXene has been used to immobilize enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine samples for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical, SEM and AFM results revealed that MXene along with chitosan is a good candidate for enzyme immobilization to fabricate a sensitive nanobiosensor. The prepared device exhibited a good analytical performance with a low detection limit of 18 nM and linear range from 0.09 to 7.8 µM. The proposed biosensing method also demonstrated high recovery index for sarcosine detection in urine samples in clinically relevant range (102.6 %).

Keywords: MXene, sarcosine, biosensor, prostate cancer

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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