OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES

1,2 BANÁŠ Dominik
Co-authors:
2 RYCHLÝ Ondřej 3 SALMISTRARO Stefano 2,4,5 AKSU Ahmet Davut 6 KRZYŽÁNKOVÁ Miroslava 2,4 KIZEK Rene
Institutions:
1 Department of Biochemistry, Masaryk University, Brno, Czech Republic, EU, dominik.banass@gmail.com
2 Department of Research and Development, ENVI-ECO-NANOLIFE, s.r.o., Prague, Czech Republic, EU
3 University of Bologna, Ozzano dell' Emilia – Bologna, Italy, EU
4 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic, EU
5 Bezmialem Vakıf Üniversites, Istanbul, Turkey
6 Veterinary Research Institute, Brno, Czech Republic, EU, krzyzankova@vri.cz
Conference:
12th International Conference on Nanomaterials - Research & Application, Brno, Czech Republic, EU, October 21 - 23, 2020
Proceedings:
Proceedings 12th International Conference on Nanomaterials - Research & Application
Pages:
324-329
ISBN:
978-80-87294-98-7
ISSN:
2694-930X
Published:
28th December 2020
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
680 views / 276 downloads
Abstract

African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of specific nucleic acid of ASFV using electrochemical hybridization biosensor. Determination of DNA was conducted by AdTSV DPV a CV (potential 0 V, end potential -1.8 V, step potential 5 mV, modulation amplitude 25 mV, 0.2 M acetate buffer pH 5.0). The volume of analysed sample was 10 µL. CV signals CA (log -0.0373x, r 0.99, Ep -1.30 V) and P peak (log -0.0801x, r 0.99, Ep -1.52 V) were observed. To increase the sensitivity, a modification of ODN with CdTe was proposed. The CdTe signal was observed around the potential of -0.56 V and for modified ODNs the signal was -0.58 V. SPION were prepared to capture DNA. The interaction of DNA (PCR fragment, 280 bp) with SPION was very fast within 30 s. The technique will be further used for a microfluidic system.

Keywords: Emergency biosensor, nucleic acid, oligonucleotide, quantum dots, super paramagnetic nanoparticle

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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