CELLULAR UPTAKE OF GRAPHENE ACID BY HEL AND HELA CELLS STUDIED BY UV RAMAN SPECTROSCOPY.

1 BALZEROVÁ Anna
Co-authors:
1 POLÁKOVÁ Kateřina 1 MALINA Tomáš 1 BELZA Jan 1 RANC Václav 1 ZBOŘIL Radek
Institution:
1 Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17 Listopadu 12, CZ-77146 Olomouc, Czech Republic, EU, anna.balzerova@upol.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
566-571
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
610 views / 195 downloads
Abstract

The last few years have witnessed rapid development of biological and medical applications of graphene-based materials in drug delivery, biosensing, and bioimaging. Graphene acid is a novel graphene-based material with many interesting properties, including colloidal stability and biocompatibility [1]. However, mechanisms of its cellular uptake are not yet fully understood, and their study is crucial for further applications of the material. Study of a cell - nanomaterial interactions of the graphene-based nano materials is commonly performed using fluorescence imaging in a combination with respective fluorescent tags. However, labelling could potentially influence the material key properties, and new ways for label-free imaging are thus demanded. Here we developed a methodology for a label-free imaging of a cellular uptake of graphene acid by cancer (HeLa) and non-cancerous (HEL) cell lines based on Raman microscopy equipped with UV excitation laser.

Keywords: Graphene acid, Raman, imaging, cells

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Scroll to Top