CELLULAR UPTAKE OF GRAPHENE ACID BY HEL AND HELA CELLS STUDIED BY UV RAMAN SPECTROSCOPY.

1 BALZEROVÁ Anna
Co-authors:
1 POLÁKOVÁ Kateřina 1 MALINA Tomáš 1 BELZA Jan 1 RANC Václav 1 ZBOŘIL Radek
Institution:
1 Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17 Listopadu 12, CZ-77146 Olomouc, Czech Republic, EU, anna.balzerova@upol.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
566-571
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
46 views / 9 downloads
Abstract

The last few years have witnessed rapid development of biological and medical applications of graphene-based materials in drug delivery, biosensing, and bioimaging. Graphene acid is a novel graphene-based material with many interesting properties, including colloidal stability and biocompatibility [1]. However, mechanisms of its cellular uptake are not yet fully understood, and their study is crucial for further applications of the material. Study of a cell - nanomaterial interactions of the graphene-based nano materials is commonly performed using fluorescence imaging in a combination with respective fluorescent tags. However, labelling could potentially influence the material key properties, and new ways for label-free imaging are thus demanded. Here we developed a methodology for a label-free imaging of a cellular uptake of graphene acid by cancer (HeLa) and non-cancerous (HEL) cell lines based on Raman microscopy equipped with UV excitation laser.

Keywords: Graphene acid, Raman, imaging, cells

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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