The last few years have witnessed rapid development of biological and medical applications of graphene-based materials in drug delivery, biosensing, and bioimaging. Graphene acid is a novel graphene-based material with many interesting properties, including colloidal stability and biocompatibility . However, mechanisms of its cellular uptake are not yet fully understood, and their study is crucial for further applications of the material. Study of a cell - nanomaterial interactions of the graphene-based nano materials is commonly performed using fluorescence imaging in a combination with respective fluorescent tags. However, labelling could potentially influence the material key properties, and new ways for label-free imaging are thus demanded. Here we developed a methodology for a label-free imaging of a cellular uptake of graphene acid by cancer (HeLa) and non-cancerous (HEL) cell lines based on Raman microscopy equipped with UV excitation laser.Keywords: Graphene acid, Raman, imaging, cells
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