MICRONUCLEI LEVELS IN NANOCOMPOSITES PRODUCTION WORKERS: INTERPRETATION OF RESULTS FROM TWO YEARS OF MONITORING

1 RÖSSNEROVÁ Andrea
Co-authors:
2 PELCLOVÁ Daniela 3 ŽDÍMAL Vladimír 1 RÖSSNER Pavel Jr. 1 ELZEINOVÁ Fatima 1 VRBOVÁ Kristýna 1 TOPINKA Jan 3 SCHWARZ Jaroslav 3 ONDRÁČEK Jakub 3 KOŠTEJN Martin 2 KOMARC Martin 2 VLČKOVÁ Štěpánka 2 FENCLOVÁ Zdenka 2 LISCHKOVÁ Lucie 4 DVOŘÁČKOVÁ Štěpánka
Institutions:
1 Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic, EU, andrea.rossnerova@iem.cas.cz, rossner.pavel@iem.cas.cz, fatima.elzeinova@iem.cas.cz, kristyna.vrbova@iem.cas.cz, jan.topinka@iem.cas.cz
2 Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic, EU, daniela@pelclova.cz, komarc@ftvs.cuni.cz, Stepanka.Vlckova@vfn.cz, zdenka.fenclova@lf1.cuni.cz, Lucie.Lischkova@vfn.cz
3 Institute of Chemical Process Fundamentals, Czech Academy of Sciences, Prague, Czech Republic, EU,zdimal@icpf.cas.cz, schwarz@icpf.cas.cz, ondracek@icpf.cz, kostejn@icpf.cz
4 Technical University in Liberec, Liberec, Czech Republic, EU, stepanka.dvorackova@tul.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
554-559
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
25 views / 12 downloads
Abstract

Micronucleus assay, which has been used for almost sixty years, is one of the basic methodological approaches of genetic toxicology. This is also evident from more than 10,500 references found in PubMed database. These studies concern the evaluation of cytotoxicity and genotoxicity of numerous chemicals and in the last decade also nanomaterials (NM) and nanoparticles (NP). Surprisingly, no relevant human studies focused on MN formation following the exposure to NP have been reported.In our study we analyzed 4x (in September 2016 and 2017; pre-shift and post-shift each year) samples in a group of workers, working for almost 18 years in nanocomposites research, and matched controls. Detail aerosol exposure monitoring of particulate matter (PM) including nano-sized fractions was completed during working shift in a sampling day. The micronucleus assay using Pan-Centromeric Chromosome Paint was applied to recognize, beside the frequency of total micronuclei (MN) in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks), including the centromere positive (CEN+) and centromere negative (CEN-) micronuclei.The monitoring data showed differences in the risk of exposure to NP related to individual working processes, as well as differences in chemical composition of nano-fraction. Cytogenetic results demonstrated consistently (both years): (i) possible adaptation to long-term exposure of NP (related to total frequency of MN), (ii) short-term (2.5 h) exposure could be a reason for the aberration increase, particularly a chromosomal losses (aneugenic effect).

Keywords: Genotoxicity, human, micronucleus assay, nanoparticles, pan-centromeric FISH
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