PDMS microchambers and microchannels were evaluated in many studies of the specific in vitro cultivation. Modern microtopographical methods bring new possibilities how to prepare transparent planar blocks with isotropic lamellas. On the other hand, the planar nanofiber sheets are another type of modern culture substrate. Neonatal myocytes and the cardiac muscle cell lineage HL-1 were used for tests of the cell adherence, cell survival and cell orientation on pure or modified polydimethylsiloxane (PDMS) or polycaprolactone (PCL). PDMS with fibronectin coating was shown to be the material with the best cell adherence, viability and isotropic cell orientation from all tested variants.Keywords: Nanofibers, microlithographical methods, in vitro models, tissue engineering, cardiomyocytes
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