THE USE OF BIOACTIVE COMPOUNDS AND THEIR ENCAPSULATION INTO LIPOSOMES TO INCREASE THE EFFECTIVENESS AND CONTROL RELEASE

1 HOOVÁ Julie
Co-authors:
1 VYSOKÁ Marie 1 DZURICKÁ Lucia 1 MATOUŠKOVÁ Petra 1 MÁROVÁ Ivana
Institution:
1 Brno University of Technology, Faculty of Chemistry, Materials Research Centre, Purkyňova 118, 612 00 Brno, Czech Republic, xchoova@fch.vut.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
386-391
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
5 views
Abstract

Extracts from naturally occurred sources, such as plants or microorganisms, have been recently tested for their interesting properties that can be used in food or cosmetic industry.The aim of this work is to encapsulate microbial compounds, some plant extracts and also model hydrophilic and hydrophobic substances. Chosen components were encapsulated into liposomes.Particle size and distributions of liposomes were analyzed by dynamic light scattering and their stability by using zeta potential. Spectrophotometric methods were used to measure encapsulation efficiency and to characterize the chosen plant extracts by its amount of total polyphenols, flavonoids and antioxidant activity.Chosen samples were analyzed for their antimicrobial activity and cytotoxicity. Serratia marcescens, Escherichia coli as gram-negative bacterias, Micrococcus luteus as gram-positive bacteria and fungal strain (Candida glabrata) were used to test antimicrobial activity. Human keratinocytes and mouse melanoma cells were used to cytotoxicity determination.It has been proved that encapsulation techniques help slowly release the active compounds from plant extracts and transport them to targeted location. Antimicrobial effect was also seen before and after encapsulation into liposomes. Moreover, none of the extracts showed evidence of cytotoxicity.

Keywords: Encapsulation, liposomes, polyphenols, antioxidant activity, antimicrobial activity, cytotoxicity
Scroll to Top