UNIVERSAL PLATFORM FOR SERS DETECTION OF “NORMAL” AND “TUMOR” CELLS CULTIVATION MEDIA

1,2 ERZINA Mariia
Co-authors:
1 TRELIN Andrii 1,2 GUSELNIKOVA Olga 1,2 KALACHYOVA Yevgeniya 1 PERMINOVA Anastasia 2 POSTNIKOV Pavel 1 ŠVORČÍK Vaclac 1,2 LYUTAKOV Oleksiy
Institutions:
1 VŠCHT - University of Chemistry and Technology Prague, Prague, Czech Republic, EU, lyutakoo@vscht.cz
2 Tomsk Polytechnic University, Research School of Chemistry and Applied Biomedical Sciences, Toms, RF
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
354-359
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
5 views
Abstract

Surface-enhanced Raman scattering (SERS) has become an attractive analytical tool for recognition of various biomolecules and bio-objects. In this work, we created a universal platform, based on surface modified gold multibranched nanoparticles (AuMs), plasmon coupling between surface plasmon polariton waves (SPP) and localized surface plasmon (LSP), and utilization of advanced statistical methods for analysis and estimation of SERS spectra, measured on the „tumor“ cells cultivation media. At the first stage, we used the AuMs which have an extremely high number of plasmonic hot spots per single nanoparticle and provide excellent SERS signal intensification. The surface of AuMs was decorated with charged organic functional moieties to entrap various (bio) molecules from targeted solution. Chemical and morphological structures of the prepared nanoparticles were examined with different techniques such as SEM-EDX and Raman spectroscopy. The decorated AuMs were placed in the cultivation media of melanoma and fibroblast lines in vitro. After interaction with cultivation media the AuMs were drop-deposited on the periodical gold grating, to achieve SPP-LSP coupling and even more enhance the SERS signal from entrapped (bio)molecules. Raman spectra were collected from several kinds of melanoma cells lines and evaluated using the developed mathematical route.

Keywords: Gold nanoparticles, SERS detection, cultivation media, arenediazonium tosylates
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