AN APPROPRIATE METHOD FOR ASSESSING HYDROGEL PORE SIZES BY CRYOSEM

1 ADAMKOVA Katerina
Co-authors:
2 TRUDICOVA Monika 1 HRUBANOVA Kamila 2 SEDLACEK Petr 1 KRZYZANEK Vladislav
Institutions:
1 The Czech Academy of Sciences, Institute of Scientific Instruments, v. v. i., Kralovopolska 147, 612 64, Brno, Czech Republic, krzyzanek@isibrno.cz
2 Brno university of technology, FCH, Purkynova 464/118, 612 00, Brno, Czech Republic, sedlacek-p@fch.vut.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
415-420
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
15 views / 3 downloads
Abstract

The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryoSEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within.Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. CryoSEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations; moreover, it showed more detailed and realistic structure.

Keywords: Hydrogel, agarose, cryoSEM, freezing methods, image analysis
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