DETECTION OF PHYTOESTROGENS BY CAPILLARY ELECTROPHORESIS IN COMBINATION WITH ISOLATION BY MAGNETIC PARTICLES MODIFIED BY MOLECULARLY IMPRINTED POLYMER

1 ZELNICKOVA Jaroslava
Co-authors:
1 VLCNOVSKA Marcela 1 ZIBEKOVA Lucia 1 ZEMANKOVA Kristyna 1 BACOVA Romana 1 KOLACKOVA Martina 1 VACULOVICOVA Marketa
Institution:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, CZ-613 00 Brno, Czech Republic, EU, zemankova.kristyna@seznam.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
437-441
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
20 views / 9 downloads
Abstract

Phytoestrogens are assumed to play an important role in the prevention of tumor or heart disease, and osteoporosis. Soy food is the most significant source of these compounds present as acetylglucosides, malonylglucosides and/or methyl ethers. The most commonly used methods for phytoestrogen determination include chromatographic or electrophoretic separation techniques.In this work, the benefits of coupling of capillary electrophoresis with molecularly imprinted polymers for analysis of phytoestrogens are shown. Polydopamin imprinted layer formed on the surface of magnetic particles enables efficient extraction/isolation of model compounds (genistein and biochanin A) from milk sample and subsequent microcolumn separation with absorbance detection enables to distinguish nonspecifically bound interferents.

Keywords: Molecularly imprinted polymers, genistein, biochanin A, micellar electrokinetic chromatography
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