The synthesis of TiO2 nanoparticles (NPs) under various temperature treatments was described and TiO2 NPs was subsequently tested as an antibacterial agent. Scanning electron microscopy (SEM), Raman spectroscopy, Dynamic light scattering (DLS) were used to confirm structure of TiO2 NPs and detect differences between individual batches treated with different temperature. Antibacterial properties were tested on Escherichia coli (E. coli). TiO2 NPs as photocatalyst was incubated with bacterial cells under ambient light. Changes in temperature treatment can affect diameter size and crystal structure of TiO2 NPs as well as its antibacterial properties.Keywords: Titanium dioxide, pathogenic bacteria, nanoparticles, antibacterial properties, E.coli, nanomaterial
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