MAGNETIC MOLECULARLY IMPRINTED POLYMERS USED FOR STAPHYLOCOCCUS AUREUS ISOLATION AND DETECTION

1 BEZDEKOVA Jaroslava
Co-authors:
1 HUTAROVA Jitka 1 VACULOVICOVA Marketa
Institution:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, CZ-613 00 Brno, Czech Republic, EU, jzelnickova@seznam.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
432-436
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
5 views
Abstract

A sensitive detection of microorganisms plays an important role in prevention and identification of infectious and deadly diseases. However, conventional methods of bacterial detection are time-consuming, laborious or expensive. A promising way to overcome these problems is offered by the strategy of molecularly imprinted polymers (MIPs), which uses molecular templates to create selective binding sites in cross-linked polymer layers. By this approach, it is possible to prepare materials tailored for recognition of target analyte taking to account the detection method available. In this work, the novel sensitive method for Staphylococcus aureus isolation and detection was investigated. This method utilized the non-covalent molecular imprinting technique employing dopamine as a functional monomer. A layer of molecularly imprinted polymer was created on a surface of magnetic particles (MPs). MPs were chosen due to the ability to pre-concentrate bacteria from large sample volumes and thereby allow for the extremely sensitive detection.

Keywords: Molecularly imprinted polymers, bacteria, dopamine, fluorescence
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