THERANOSTIC TOOL FOR TARGETING OF BACTERIAL CELLS BASED ON SILVER NANOPARTICLES

1 GARGULÁK Michael
Co-authors:
1 ČÍŽEK Matouš 2,5 DOČEKALOVÁ Michaela 2 KEPINSKA Marta 1,3 FERREIRA DA SILVA Andre 1 SUROVÍKOVÁ Aneta 2 UHLÍŘOVÁ Dagmar 2 STAŇKOVÁ Martina 1,5 RUTTKAY-NEDECKÝ Branislav 5 VAVŘINÍK Aleš 5 SOCHOR Jiří 6 NGUYEN Hoai Viet 1,2,4,5 KIZEK René
Institutions:
1 University of Veterinary and Pharmaceutical Sciences Brno, Pharmaceutical Faculty, Brno, Czech Republic, EU, kizek@sci.muni.cz
2 Prevention Medicals, Studenka-Butovice, Czech Republic, EU, uhlirova@preventionmedicals.cz
3 University of Lisboa, Faculty of Pharmacy Avenida Professor Gama Pinto, 1649-003, Lisboa - Portugal, EU, andre_ferreira.silva@hotmail.com
4 Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland, EU, zalewska.m@gmail.com
5 Mendel University in Brno, Faculty of Horticulture, Department of Viticulture and Enology, Lednice, Czech Republic, EU, sochor.jirik@seznam.cz
6 Research Center for Environmental Monitoring and Modeling, VNU University of Science, Hanoi, Vietnam, nguyenviethoai@hus.edu.vn
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
370-375
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
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Abstract

Infectious diseases are known to be a constant threat to human health also in the beginning of the 21st century. The rapid growth of the human population has led to the agricultural intensification, which has caused an increase in resistant bacterial species in the environment. The aim of the project was to design and verify a theranostic tool combining the diagnostic and therapeutic parts for targeting the bacterial cells. The construct was composed of: A) silver nanoparticles prepared by green synthesis (AgNPsGS); B) antibiotic encapsulated in apoferritin (APO); C) modified magnetic gold nanoparticles (AuNPs). The effect of theranostic nanotransporter on prokaryotic cell culture was model-monitored by altering their growth characteristics (OD monitoring). During the project, 20 various types of AgNPsGS were prepared. The best antibacterial effect (85% growth inhibition of S. aureus) with the minimal inhibitory concentration (MIC50) of 0.4 μg/mL was observed by AgNPsGS4 (Thymus serpyllum L.). SPIONs (20 mg/mL, magnetic AuNPs) were modified with antibody (1 mg/mL IgY) and oxidized graphene sheets (1 mg/mL). The EDC/NHS polymer (2,2 mM EDC, 4 mM NHS, 3 h, 37 °C) was used for binding. The apoferritin (SPIONs/Au/APO) was attached to the SPION/AuNPs. In the SPIONs/Au/APO cavity (12 nm), the antibiotic doxorubicin (2 μM DOXO, the DOXO encapsulation efficiency was around 13% of the applied concentration) was model-sealed. The created SPIONs/Au/AgNPsGS4/APO/DOXO construct was applied to the S. aureus model culture. In the presence of 70 μg of SPIONs/Au/AgNPsGS4/APO/DOXO, a dramatic growth inhibition of S. aureus (95% growth inhibition and 13 mm wide inhibition zone on agar medium) was observed. The biological effect of the nanotransporter lies in ROS (reactive oxygen species) formation, DNA destruction and cell membrane damage.

Keywords: Nanomedicine, silver nanoparticles, nanoconstructs, targeted therapy
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